representative blots Search Results


dot blots of glutathione s-transferase (gst) fusion proteins representing all of the known pdz sequences  (Panomics Inc)
90
Panomics Inc dot blots of glutathione s-transferase (gst) fusion proteins representing all of the known pdz sequences
Binding of C-terminal peptides to <t>PDZ</t> domains. The binding of synthetic peptides to dot-blotted GST fusion proteins representing PDZ domains. Lane 1: a peptide that models the C-terminus of CD81 Lane 2: a peptide without the putative PDZ binding domain. Lane 3: the binding of a scrambled protein. Specific binding was observed for the Sap97 domain 3, the protein-tyrosine phosphatase domain 1 (PTP-D1), and the X11L2 protein domain 1 (Mint-D1). Each PDZ domain is spotted with two concentrations of protein: 400 ng (upper spot) and 80 ng (lower spot). GST <t>represents</t> <t>glutathione</t> S-transferase.
Dot Blots Of Glutathione S Transferase (Gst) Fusion Proteins Representing All Of The Known Pdz Sequences, supplied by Panomics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dot blots of glutathione s-transferase (gst) fusion proteins representing all of the known pdz sequences/product/Panomics Inc
Average 90 stars, based on 1 article reviews
dot blots of glutathione s-transferase (gst) fusion proteins representing all of the known pdz sequences - by Bioz Stars, 2026-03
90/100 stars
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representative western blot analysis  (Promega)
90
Promega representative western blot analysis
Binding of C-terminal peptides to <t>PDZ</t> domains. The binding of synthetic peptides to dot-blotted GST fusion proteins representing PDZ domains. Lane 1: a peptide that models the C-terminus of CD81 Lane 2: a peptide without the putative PDZ binding domain. Lane 3: the binding of a scrambled protein. Specific binding was observed for the Sap97 domain 3, the protein-tyrosine phosphatase domain 1 (PTP-D1), and the X11L2 protein domain 1 (Mint-D1). Each PDZ domain is spotted with two concentrations of protein: 400 ng (upper spot) and 80 ng (lower spot). GST <t>represents</t> <t>glutathione</t> S-transferase.
Representative Western Blot Analysis, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/representative western blot analysis/product/Promega
Average 90 stars, based on 1 article reviews
representative western blot analysis - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Binding of C-terminal peptides to PDZ domains. The binding of synthetic peptides to dot-blotted GST fusion proteins representing PDZ domains. Lane 1: a peptide that models the C-terminus of CD81 Lane 2: a peptide without the putative PDZ binding domain. Lane 3: the binding of a scrambled protein. Specific binding was observed for the Sap97 domain 3, the protein-tyrosine phosphatase domain 1 (PTP-D1), and the X11L2 protein domain 1 (Mint-D1). Each PDZ domain is spotted with two concentrations of protein: 400 ng (upper spot) and 80 ng (lower spot). GST represents glutathione S-transferase.

Journal: Molecular Vision

Article Title: The developmental regulation of CD81 in the rat retina

doi:

Figure Lengend Snippet: Binding of C-terminal peptides to PDZ domains. The binding of synthetic peptides to dot-blotted GST fusion proteins representing PDZ domains. Lane 1: a peptide that models the C-terminus of CD81 Lane 2: a peptide without the putative PDZ binding domain. Lane 3: the binding of a scrambled protein. Specific binding was observed for the Sap97 domain 3, the protein-tyrosine phosphatase domain 1 (PTP-D1), and the X11L2 protein domain 1 (Mint-D1). Each PDZ domain is spotted with two concentrations of protein: 400 ng (upper spot) and 80 ng (lower spot). GST represents glutathione S-transferase.

Article Snippet: We purchased dot blots of glutathione S-transferase (GST) fusion proteins representing all of the known PDZ sequences from Panomics.

Techniques: Binding Assay

Proteins associated with CD81. Cultured rat retinal pigment epithelium (RPE) cells were solubilized in 1% Brij 97 and immunoprecipitated with mouse anti-rat CD81 antibody. The sample of proteins was then separated by SDS PAGE, transferred to nitrocellulose, and probed with antibodies directed against CD81 (lane A), EBP50 (lane B), Ezrin (lane C), and Sap97 (lane D). Note that CD81 coimmunoprecipitated with EBP50, Ezrin, and Sap97. In control experiments, antibodies directed against the transferrin receptor (CD71) were used to immunoprecipitate proteins from cultured RPE. When these samples were probed for EBP50 (lane E) or Sap97 (lane F), these two PDZ proteins were not observed. Molecular weight markers are indicated to the left in kDa.

Journal: Molecular Vision

Article Title: The developmental regulation of CD81 in the rat retina

doi:

Figure Lengend Snippet: Proteins associated with CD81. Cultured rat retinal pigment epithelium (RPE) cells were solubilized in 1% Brij 97 and immunoprecipitated with mouse anti-rat CD81 antibody. The sample of proteins was then separated by SDS PAGE, transferred to nitrocellulose, and probed with antibodies directed against CD81 (lane A), EBP50 (lane B), Ezrin (lane C), and Sap97 (lane D). Note that CD81 coimmunoprecipitated with EBP50, Ezrin, and Sap97. In control experiments, antibodies directed against the transferrin receptor (CD71) were used to immunoprecipitate proteins from cultured RPE. When these samples were probed for EBP50 (lane E) or Sap97 (lane F), these two PDZ proteins were not observed. Molecular weight markers are indicated to the left in kDa.

Article Snippet: We purchased dot blots of glutathione S-transferase (GST) fusion proteins representing all of the known PDZ sequences from Panomics.

Techniques: Cell Culture, Immunoprecipitation, SDS Page, Control, Molecular Weight